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An optimized magnetite microparticle-based phosphopeptide enrichment strategy for identifying multiple phosphorylation sites in an immunoprecipitated protein

To further improve the selectivity and throughput of phosphopeptide analysis for the samples from real-time cell lysates, here we demonstrate a highly efficient method for phosphopeptide enrichment via newly synthesized magnetite microparticles and the concurrent mass spectrometric analysis.

出版机构:Elsevier Science

刊物名称:Analytical Biochemistry

出版时间:2011

ISSN:0003-2697

数据源:报刊文摘

Sites on phospholipase D2 phosphorylated by PKCα

The phosphorylation sites in phospholipase D2 (PLD2) induced by activation of protein kinase C? (PKC?) in COS 7 cells were analyzed by mass spectrometry. Ser134, 146, and 243, and Thr72, 99/100, and 252 were identified.

出版机构:Elsevier Science

刊物名称:Biochemical and Biophysical Research Communications

出版时间:2005

ISSN:0006-291X

数据源:报刊文摘

Linking the proteins—Elucidation of proteome‐scale networks using mass spectrometry

Proteomes are intricate. Typically, thousands of proteins interact through physical association and post?translational modifications (PTMs) to give rise to the emergent functions of cells. Understanding these functions requires one to study proteomes as ?systems?

出版机构:Wiley Subscription Services, Inc., A Wiley Company

刊物名称:Mass Spectrometry Reviews

出版时间:2011

ISSN:0277-7037

数据源:报刊文摘

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) - an Introduction for Biologists

Since its introduction in 2002 'stable isotope labeling by amino acids in cell culture' (SILAC) has become a major tool for quantitative proteomics. Stable isotopes are incorporated into proteins by introduction of labeled amino acids in growth medium.

出版机构:Bentham Science Publishers

刊物名称:Current Proteomics

出版时间:2011

ISSN:1570-1646

数据源:报刊文摘

Bacterial Protein-Tyrosine Kinases

Bacteria and Eukarya share essentially the same family of protein-serine/threonine kinases, also known as the Hanks-type kinases. However, when it comes to protein-tyrosine phosphorylation, bacteria seem to have gone their own way.

出版机构:Bentham Science Publishers

刊物名称:Current Proteomics

出版时间:2010

ISSN:1570-1646

数据源:报刊文摘

Recent Applications of Phosphoproteomics

Protein phosphorylation plays a key role in the regulation of most cellular processes and the disorder of this process often leads to illness or even cancers.

出版机构:Bentham Science Publishers

刊物名称:Current Proteomics

出版时间:2010

ISSN:1570-1646

数据源:报刊文摘

Analysis of recombinant phosphoprotein complexes with complementary mass spectrometry approaches

The baculovirus expression vector system is recognized as a powerful and versatile tool for producing large quantities of recombinant proteins that cannot be obtained in Escherichia coli.

出版机构:Elsevier Science

刊物名称:Analytical Biochemistry

出版时间:2010

ISSN:0003-2697

数据源:报刊文摘

Subcellular phosphoproteomics

Protein phosphorylation represents one of the most extensively studied post?translational modifications, primarily due to the emergence of sensitive methods enabling the detection of this modification both in vitro and in vivo.

出版机构:Wiley Subscription Services, Inc., A Wiley Company

刊物名称:Mass Spectrometry Reviews

出版时间:2010

ISSN:0277-7037

数据源:报刊文摘

Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate‐affinity electrophoresis

The phosphorylation of heterogeneous nuclear ribonucleoprotein K (hnRNP K) is thought to play an important role in cell regulation and signal transduction.

出版机构:WILEY‐VCH Verlag

刊物名称:PROTEOMICS

出版时间:2010

ISSN:1615-9853

数据源:报刊文摘

Homologous desensitization of guanylyl cyclase A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern

Atrial natriuretic peptide (ANP), via its guanylyl cyclase A (GC-A) receptor and intracellular guanosine 3',5'-cyclic monophosphate production, is critically involved in the regulation of blood pressure.

出版机构:Blackwell Publishing Ltd

刊物名称:FEBS Journal

出版时间:2010

ISSN:1742-464X

数据源:报刊文摘