Binding of Calcium to Serum Albumin I. Stoichiometry and Intrinsic Association Constant at Physiological pH, Ionic Strength, and Temperature

Ultrafiltration and spectrophotometric methods have shown that calcium is bound reversibly to serum albumin by a single class of 12?1 identical, independent binding sites with an association constant = 90-100 litres/mole at 37 ?C in unbuffered solutions with pH 7.4 and ionic strength 0.15-0.16. Tea.